L-Isoleucine L-异亮氨酸

CAS 73-32-5 MFCD00064222

化学结构图

73-32-5
SMILES: CC[C@H](C)[C@H](N)C(=O)O

化学属性

Mol. FormulaC6H13NO2
Mol. Weight131.17
Density1.035
Melting Point288°C
TSCAYes
Solubility41.2 G/L (50 ºC)
Flash Point168-170°C
Appearance D 为白色结晶或结晶性粉末， 无臭， 味微苦。 mp284℃（分解）， 168-170℃升华。 在水中的溶解度为4.12%， 极难溶于乙醇和乙醚， [α] 25 powder +12.4 (C=0.5-2.0g/ml, H2O)， 等电点pI为6.02。 +39.5 (C=0.5-2.0g/ml, 5mol/L HCl), [α]
Refractive index40.5 ° (C=4, 6mol/L HCl)
Boiling Point225.8±23.0 °C at 760 mmHg

别名和识别编码

Chemical NameL-Isoleucine
Synonym L-Isoleucine {LY} L-Isoleucine {} {LY} L-Isoleucine {} {} {LY} L-Isoleucine {} {} {} {LY} L-Isoleucine {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} L-Isoleucine {} {} {} {} {} {} {} {} {} {} {} {}
CAS Number73-32-5
EC Number200-798-2
Beilstein Registry Number1721792
MDL NumberMFCD00064222
PubChem Substance ID6306
Reaxys-RN1721792
Merck Number5179
Chemical Name TranslationL-异亮氨酸
Wiswesser Line NotationQVYZY2&1 -L
LabNetwork Molecule IDLN00195374
InChIInChI=1S/C6H13NO2/c1-3-4(2)5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t4-,5-/m0/s1
Canonical SMILESN[C@@H]([C@@H](C)CC)C(O)=O
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分类

  • {SNA} All Alphabetically Sorted, Amino Acids, Analytical Standards, Analytical/Chromatography, Certified Reference Materials (CRMs), Chromatography, Flavors and Fragrances, Fluka CRM for chromatography (neats), Food & Beverage Standards, G-L, Organic CRMs Alphabetical, Organic CRMs TraceCERT, Substance Classification
  • {SNA} Amino Acids, Analytical/Chromatography, Biochemicals and Reagents, Pharmacopoeia, Pharmacopoeia A-Z, Pharmacopoeial Amino Acids
  • {SNA} Analytical Standards, Analytical/Chromatography, Chromatography, EP Standards, EP Standards I - K, Pharmaceu

产品应用

  • 生化研究。细菌学及组织培养。

相关文献及参考

  • Beilstein:4,454
  • Short: III/10a
  • Short: IV/1b
  • Short: IV/20B
  • [1]. Arkhipov SG, et al. New hydrophobic L-amino acid salts: maleates of L-leucine, L-isoleucine and L-norvaline. Acta Crystallogr C Struct Chem. 2015 Jul;71(Pt 7):584-92.

安全信息

Signal word Warning
GHS Symbol
WGK Germany1
Safety Statements
  • S24/25 Avoid contact with skin and eyes 避免皮肤和眼睛接触；
Risk Statements
  • R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
Precautionary statements
  • P262 Do not get in eyes, on skin, or on clothing. 不要接触眼睛，皮肤或衣服。
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RTECSNR4705000
Storage condition 2-8°C {LY} 2-8°C Store at RT. {} {LY} 2-8°C {} {} {LY} 2-8°C {} {} {} {LY} 2-8°C {} {} {} {} {LY} 2-8°C {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 6822 mg/kg
TOXIC EFFECTS :
   Lungs, Thorax, or Respiration - dyspnea
   Nutritional and Gross Metabolic - body temperature decrease
REFERENCE :
   ABBIA4 Archives of Biochemistry and Biophysics.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802)  V.31-    1951-  Volume(issue)/page/year:
   58,253,1955

TYPE OF TEST            : Sister chromatid exchange
TEST SYSTEM             : Human Lymphocyte
DOSE/DURATION           : 10 mg/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 372,75,1996

Hazard statements

其他信息

  • 方法一:α-乙酰乳酸合成酶[培养液（菌种培养）]→菌种放大培养[发酵]→[30-31℃, 60h]发酵液[过滤]→[加热]上层液[草酸、硫酸（酸化）]→[过滤]滤液[H + 732树脂]→[（吸附分离）]洗脱液[浓缩]→[减压蒸馏]浓缩液[NH3水（中和）]→沉淀[水（精制）]→结晶[105℃烘干]→L-异亮氨酸成品 菌种培养 种子培养基组成为葡萄糖2%， 尿素0.3%, 玉米浆2.5%，豆饼水解液0.1% (以干豆饼计)， 调pH6.5, 保温118-120℃， 灭菌30min。 一级种子培养。 在1000ml锥形瓶中加入上述培养基200ml, 接种一环牛肉膏斜面AS（即α-乙酰乳酸合成酶）1.998菌种, 30℃培养, 冲程7cm, 频率105次/min, 16h。 二级种子培养。 二级种子培养基和一级种子培养基相似， 另加菜籽油0.4%， 接种量3.5%，培养8h， 如此逐级放大培养足够量的菌种。 发酵、酸化 发酵培养液是由硫酸铵4.5%, 豆饼水解液0.4%， 玉米浆2.0%, 碳酸钙4.5%, 调pH至7.2， 淀粉水解还原糖初糖浓度11.5所组成的。然后在5m 3 发酵罐中添加上述发酵培养液3t， 加热保温118-120℃， 控制压力1.1×10 5 Pa， 灭菌30min后，立即通冰盐水冷却至25℃， 接入1%菌种， 以180r/min转速搅拌保温30-31℃，以0.21L/min通气量发酵60h，在24-50h之间不断添加尿素至0.6%， 氨水至0.27%。 待发酵60h后， 将发酵液加热至100℃， 并保持10min。 冷却后过滤， 除去菌体， 滤液加工业用硫酸铵和草酸至pH3.5, 过滤取滤液。 分离、浓缩 将上述滤液以每分钟树脂量1.5%的流速流过氢型732离子交换树脂 (400mm×1000mm)后， 再用100L去离子水洗涤树脂柱， 最后以60℃，0.5mol/L的氨水， 按3L/min的流速进行洗脱， 并分步收集洗脱液。 合并pH为3-12的洗脱液于70-80℃减压蒸馏浓缩至黏稠状。 再加去离子水至原体积的1/4， 再浓缩至黏稠状， 赶氨， 如此反复三次。 脱色、浓缩、中和 将上述浓缩黏稠物加去离子水至原体积的1/4，搅拌均匀， 并加2mol/L的HCl调pH至3.5， 再加入1%的活性炭， 于70-80℃保温搅拌脱色1h, 过滤取滤液减压浓缩， 最后加入2mol/L的HCl调pH至6.0， 保温5℃，静置沉淀过夜， 抽滤取沉淀于105℃下洪干， 得半成品。 精制 取10kg半成品， 加入浓盐酸8L, 去离子水20L， 加热搅拌使其溶解后， 再加入10kg NaCl至饱和，
  • 图谱信息:L-2-氨基-3-甲基戊酸(73-32-5)质谱(MS) L-2-氨基-3-甲基戊酸(73-32-5)红外图谱(IR1) L-2-氨基-3-甲基戊酸(73-32-5)Raman光谱 L-2-氨基-3-甲基戊酸(73-32-5)红外图谱(IR2) L-2-氨基-3-甲基戊酸(73-32-5)核磁图( 13 CNMR)
  • 所属类别二:生物化学品: 生化试剂: 氨基酸及其衍生物
  • 所属类别一:生物化学品: 氨基酸类: 中性氨基酸
  • {Chem
  • MSDS 信息:(2S,3S)-2-Amino-3-methylpentanoic acid(73-32-5).msds
  • 味苦。168～170℃升华，284℃分解。水中溶解度25℃时41.2g/L，微溶于热乙醇（w/w)和热乙醚，不溶于乙醚。
  • 方法二:以糖、氨、D-苏氨酸为原料，经粘质赛氏杆菌(Serratia marcescens)发酵而得，发酵液最终浓度0.5～1.5g/100ml。或以糖、氨、α-氨基丁酸为原料，用黄色小球菌(Micrococcus flavum)或枯草杆菌(Bacillus subtilis, Aerobactor)发酵而得，发酵液最终浓度可达1.0～1.5g/100ml。
  • 用途一:用作氨基酸注射液、复合氨基酸输液、食品添加剂
  • 下游产品:L-亮氨酸 --> BOC-L-异亮氨酸 --> 2-甲氧基-3-仲丁基吡嗪 --> D-异亮氨酸
  • 用途五:营养增补剂。L-异亮氨酸为人体必须氨基酸之一，每天最低需要量约0.7g，但过量食用将与亮氨酸产生拮抗作用，有碍发育。本品可强化各种食品，如小麦粉、麦谷蛋白
  • L-异亮氨酸价格(试剂级):

系列性分类

  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Amino Acids 氨基酸
  1. Application Areas 应用领域分类
  2. Drug Discovery 新药研发
  3. Pharmacopoeia 药典
  4. Pharmacopoeia A-Z - Pharmacopoeia
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Amino Acids 氨基酸
  4. BioUltra - Amino Acids
  1. Application Areas 应用领域分类
  2. Flavors and Fragrances 香精香料
  3. Alphabetical Listings - Flavors and Fragrances
  4. I-L - Alphabetical Listings
  1. Life Science 生命科学
  2. Metabolomics 代谢组学
  3. Metabolic Libraries - Metabolomics
  4. Amino Acid Library - Metabolic Libraries
  1. Life Science 生命科学
  2. Nutrition Research 营养研究
  3. Phytochemicals by Plant (Food/Spice/Herb) - Nutrition Research
  4. Allium cepa (Onion) - Phytochemicals by Plant (Food/Spice/Herb)
  1. Life Science 生命科学
  2. Nutrition Research 营养研究
  3. Phytochemicals by Plant (Food/Spice/Herb) - Nutrition Research
  4. Allium sativum (Garlic) - Phytochemicals by Plant (Food/Spice/Herb)
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Amino Acids 氨基酸
  4. Modified Amino Acids - Amino Acids
  5. I - Z - Modified Amino Acids
  1. Analytical & Chromatography 分析化学与色谱
  2. Analytical Standards
  3. Chromatography - Analytical Standards
  4. Pharmaceutical Standards - Chromatography
  5. Pharmaceutical Secondary Standards - Pharmaceutical Standards
  1. Life Science 生命科学
  2. Metabolomics 代谢组学
  3. Metabolic Pathways - Metabolomics
  4. Metabolites and Cofactors on the Metabolic Pathways Chart - Metabolic Pathways
  5. Amino Acid - Metabolites and Cofactors on the Metabolic Pathways Chart
  1. Chemical Synthesis 化学合成
  2. Chemical Biology 生物化学
  3. Peptide Chemistry 多肽化学
  4. Amino Acid Derivatives 氨基酸及其衍生物
  5. Isoleucine - Amino Acid Derivatives
  1. Chemical Synthesis 化学合成
  2. Chemical Biology 生物化学
  3. Peptide Chemistry 多肽化学
  4. Amino Acid Derivatives 氨基酸及其衍生物
  5. Isoleucine - Amino Acid Derivatives
  1. Analytical & Chromatography 分析化学与色谱
  2. Analytical Standards
  3. Chromatography - Analytical Standards
  4. Food & Beverage Standards - Chromatography
  5. Nutraceuticals - Food & Beverage Standards
  6. Flavors and Fragrances - Nutraceuticals
  7. G-L - Flavors and Fragrances
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Amino Acids
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Albumins and Transport Proteins
  4. Albumin
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Metabolomics
  4. Metabolic Pathways
  5. Metabolites and Cofactors on the Metabolic Pathways Chart
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Enzymes, Inhibitors, and Substrates
  4. Metabolomics
  5. Metabolic Pathways
  6. Metabolites and Cofactors on the Metabolic Pathways Chart
  1. Medical Research Areas
  2. Metabolism
  3. Endogenous Metabolite  (内源性代谢物)