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分类

• {SNA} 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Dissociation, Cell Dissociation and Cell Lysis, Cell Signaling Enzymes, Cell Signaling and Neuroscience, Collagenases, Core Bioreagents, Crude Collagenase, Use-Tested, Cytoskeleton and Extracellular Matrix, Enzyme Class Index, Enzymes, Enzymes for Diagnostic Kit Manufacturing, Enzymes, Inhibitors, and Substrates, Extracellular Matrix, Extracellular Matrix Peptides and Proteins, Histology, MMP Enzymes, Matrix Metalloproteinases Products, Proteases, Proteases & Protein

产品应用

• This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.

相关文献及参考

• Templeton, N.S., et al., Cloning and characterization of human tumor cell interstitial collagenase. Cancer Res. 50 , 5431-5437, (1990) 摘要
• Cawston, T.E., Barrett, N.D., et al., Interstitial collagenase. Handbook of Proteolytic Enzymes San Diego , (2004), 472-480

安全信息

其他信息

• Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.