Trypsin [Porcine 1:250] TPCK-胰蛋白酶

CAS 9002-07-7 MFCD00082094

化学结构图

9002-07-7
SMILES: CC(=O)O.Cc1cc(NC(=O)C(CCCN=C(N)N)NC(=O)C(CO)NC(=O)C(Cc2ccccc2)NC(=O)OC(C)(C)C)cc2oc(=O)ccc12

化学属性

Mol. Weight-
Mol. Formula-
Melting Point115 ºC
Stability易潮湿
SolubilitySoluble in Hank's Balanced Salt Solution (35 mg/ml), HCl (1 mM), and water. Insoluble in clycerol, and alcohol

别名和识别编码

MDL NumberMFCD00082094
CAS Number9002-07-7
EC Number232-650-8
Synonym Parenzyme Parenzymol Peptidyl peptide hydrolase Trypsin from Porcine Pancreas {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {S {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {ALD} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
Chemical NameTrypsin [Porcine 1:250]
Chemical Name TranslationTPCK-胰蛋白酶
PubChem Substance ID5311489
InChIInChI=1S/C33H43N7O8.C2H4O2/c1-19-15-21(17-26-22(19)12-13-27(42)47-26)37-28(43)23(11-8-14-36-31(34)35)38-30(45)25(18-41)39-29(44)24(16-20-9-6-5-7-10-20)40-32(46)48-33(2,3)4;1-2(3)4/h5-7,9-10,12-13,15,17,23-25,41H,8,11,14,16,18H2,1-4H3,(H,37,43)(H,38,45)(H,39,44)(H,40,46)(H4,34,35,36);1H3,(H,3,4)/t23-,24-,25-;/m0./s1
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分类

  • {SNA} 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index, Cell Dissociation, Cell Dissociation (Cell Culture Tested), Cell Dissociation Enzymes, Cell Dissociation and Cell Lysis, Dissociation, Enzyme Class Index, General Stem Cell Biology, Stem Cell Isolation,

产品应用

  • Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins

相关文献及参考

  • Merck: 14,9795

安全信息

RTECSYN5075000
GHS Symbol
WGK Germany1
Precautionary statements
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
  • P284 Wear respiratory protection.? 佩戴呼吸保护装置。
  • P304+P340
  • P304+P340+P312
  • P305+P351+P338
  • P337+P313
  • P342+P311
Signal word Danger
Hazard statements
  • H315 Causes skin irritation 会刺激皮肤
  • H319 Causes serious eye irritation 严重刺激眼睛
  • H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled 吸入可能导致过敏或哮喘症状或呼吸困难
  • H335 May cause respiratory irritation 可能导致呼吸道刺激
  • H317 May cause an allergic skin reaction 可能导致皮肤过敏
Hazard Codes Xn
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Safety Statements
  • S22 Do not breathe dust 不要吸入粉尘；
  • S23 Do not breathe gas/fumes/vapour/spray (appropriate wording to be specified by the manufacturer) 不要吸入气体/烟雾/蒸汽/喷雾；
  • S24 Avoid contact with skin 避免皮肤接触；
  • S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后，立即用大量水冲洗并征求医生意见；
  • S36/37 Wear suitable protective clothing and gloves 穿戴适当的防护服和手套；
  • S45 In case of accident or if you feel unwell seek medical advice immediately (show the label where possible) 发生事故时或感觉不适时，立即求医（可能时出示标签）；
Risk Statements
  • R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
  • R42 May cause sensitisation by inhalation 吸入会产生过敏反应
Storage condition -20℃保存。 储存温度-20°C 储存温度2-8℃ 储存温度?20°C 储存温度 -20°C 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : >51 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   NIIRDN Drugs in Japan (Ethical Drugs).  (Yakugyo Jiho Co., Ltd., Tokyo,
   Japan)  Volume(issue)/page/year: -,744,1990

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 100 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   FATOAO Farmakologiya i Toksikologiya (Moscow).  For English translation, see
   PHTXA6 and RPTOAN. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.2-   
   1939-  Volume(issue)/page/year: 45(6),78,1982

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intramuscular
SPECIES OBSERVED        : Rodent - r

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 1450 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   NIIRDN Drugs in Japan (Ethical Drugs).  (Yakugyo Jiho Co., Ltd., Tokyo,
   Japan)  Volume(issue)/page/year: -,744,1990

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 36 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   FATOAO Farmakologiya i Toksikologiya (Moscow).  For English translation, see
   PHTXA6 and RPTOAN. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.2-   
   1939-  Volume(issue)/page/year: 45(6),78,1982

TYPE OF TEST            : DNA damage
TEST SYSTEM             : Rodent - hamster Fibroblast
DOSE/DURATION           : 100 ppm
REFERENCE :
   SFCRAO Collection of Papers Presented at the Annual Symposium on Fundamental
   Cancer Research. (Baltimore, MD)  V.1-29, 1946-76.  For publisher
   information, see AFCPDR.  Volume(issue)/page/year: 23,346,1970

其他信息

  • 胰蛋白酶是一种丝氨酸蛋白水解酶,属白色或微黄色冻干粉制品。它能特异性地水解碱性氨基酸精氨酸及赖氨酸羧基所组成的肽键。酶本身很容易自溶，其作用最佳的PH值为8.0～9.0。胰蛋白酶活性能被胰腺中的天然抑制剂所抑制。钙离子能延迟胰蛋白酶的自溶，并能促进胰蛋白酶原的活性。
  • 本品为经TPCK处理的胰蛋白酶，因此，胰蛋白酶活力更高，糜胰蛋白酶活性被抑制。TPCK-胰蛋白酶能够专一性地切割蛋白分子中精氨酸和赖氨酸位点，是蛋白质肽段分析重要工具酶之一。本品为白色或微黄色冻干粉制品。
  • Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.

系列性分类

  1. Research Essentials 基础科研
  2. Core Bioreagents - Research Essentials
  3. Life Science Reagents for Immunohistochemistry (IHC) - Core Bioreagents
  1. Labware 实验室仪器设备与耗材
  2. Cell Culture Supplies - Labware
  3. Scale-Up Cell Culture using Corning® Roller Bottles - Cell Culture Supplies
  4. Supplies for Scale-Up Cell Dissociation - Scale-Up Cell Culture using Corning® Roller Bottles
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Application Index - Enzymes, Inhibitors, and Substrates
  5. Proteases & Protein Sequencing - Application Index
  6. Proteases - Proteases & Protein Sequencing
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Enzyme Class Index - Enzymes, Inhibitors, and Substrates
  5. 3.x.x.x Hydrolases - Enzyme Class Index
  6. 3.4.x.x Peptidases - 3.x.x.x Hydrolases
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Analytical Enzymes - Enzymes, Inhibitors, and Substrates
  5. Trypsin - Analytical Enzymes
  6. Trypsin for General Research Applications - Trypsin
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Analytical Enzymes - Enzymes, Inhibitors, and Substrates
  5. Trypsin - Analytical Enzymes
  6. Trypsin for General Research Applications - Trypsin
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Analytical Enzymes - Enzymes, Inhibitors, and Substrates
  5. Trypsin - Analytical Enzymes
  6. Trypsin Solutions for Cell Dissociation - Trypsin
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Analytical Enzymes - Enzymes, Inhibitors, and Substrates
  5. Trypsin - Analytical Enzymes
  6. Trypsin Solutions for Cell Dissociation - Trypsin
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Proteolytic Enzymes and Substrates - Enzymes, Inhibitors, and Substrates
  5. Selective Proteolytic Enzymes - Proteolytic Enzymes and Substrates
  6. Trypsin - Selective Proteolytic Enzymes

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