• 原料直供
• 试剂商城
• 直接询价

分类

• {SA} 3.2.x.x Glycosidases,
• {SNA} 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Carbohydrate Hydrolysis, Carbohydrate and PTM Analysis, Carbohydrate hydrolysis & PTM analysis, Enzyme Class Index, Other Apoptosis Related Reagents, Other Apoptosis Related Reagents Enzymes, 生化试剂, 细胞信号转导和神经科学, 细胞生物学, 酶、抑制剂和底物
• {SNA} 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Carbohydrate Hydrolysis,
• {SNA} 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Carbohydrate Hydrolysis, Carbohydrate and PTM Analysis,

产品应用

• β-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.

相关文献及参考

• 1. D.H. Juers et al., Structural comparisons of TIM barrel proteins suggest functional and evolutionary relationships between &#946-galactosidase and other glycohydrolases Protein Sci. 8 , 122-139, (1999)
• Fowler, A.V., et al., Amino acid sequence of beta-galactosidase XI; Peptide ordering procedures and the complete sequence. J. Biol. Chem. 253 , 5521, (1978)

安全信息

TYPE OF TEST

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 4090 mg/kg
TOXIC EFFECTS :
   Behavioral - somnolence (general depressed activity)
   Behavioral - muscle weakness
REFERENCE :
   KSRNAM Kiso to Rinsho.  Clinical Report.  (Yubunsha Co., Ltd., 1-5, Kanda
   Suda-Cho, Chiyoda-ku, KS Bldg., Tokyo 101, Japan)  V.1-    1960-
   Volume(issue)/page/year: 4,725,1970

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 1470 mg/kg
TOXIC EFFECTS :
   Behavioral - somnolence (general depressed activity)
   Behavioral - muscle weakness
REFERENCE :
   KSRNAM Kiso to Rinsho.  Clinical Report.  (Yubunsha Co., Ltd., 1-5, Kanda
   Suda-Cho, Chiyoda-ku, KS Bldg., Tokyo 101, Japan)  V.1-    1960-
   Volume(issue)/page/year: 4,729,1970

TYPE OF TEST            : LD - Lethal dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : >20 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   KSRNAM Kiso to Rinsho.  Clinical Report.  (Yubunsha Co., Ltd., 1-5, Kanda
   Suda-Cho, Chiyoda-ku, KS Bldg., Tokyo 101, Japan)  V.1-    1960-
   Volume(issue)/page/year: 4,725,1970

{hazard_co

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 630 mg/kg
TOXIC EFFECTS :
   Behavioral - somnolence (general depressed activity)
   Behavioral - muscle weakness
REFERENCE :
   KSRNAM Kiso to Rinsho.  Clinical Report.  (Yubunsha Co., Ltd., 1-5, Kanda
   Suda-Cho, Chiyoda-ku, KS Bldg., Tokyo 101, Japan)  V.1-    1960-
   Volume(issue)/page/year: 4,729,1970

其他信息

• β-Galactosidase has a molecular weight of 540 kDa, and an optimum pH range of 6 to 8. Product G128643 is not suitable for immunoconjugation applications due to the presence of ammonium sulfate.
• 无臭，微甜。分子量100000～850000。在水中呈混沌液(大部分溶解)，不溶于乙醇、丙酮。不同来源的酶最适pH值在4.0～7.5不等。最适作用温度37～50℃，低温保存。可由米曲霉(Aspergillus oryzae)培养液或制干酪时所得的乳清制得。作药物，适用于婴儿各种乳糖消化不良症及腹泻等。