Luminol 鲁米诺

CAS 521-31-3 MFCD00006890

化学结构图

521-31-3
SMILES: Nc1cccc2c(=O)[nH][nH]c(=O)c12

化学属性

Mol. FormulaC8H7N3O2
Mol. Weight177.16
Melting Point325-330°C
TSCAYes
Density1.433
Solubility易溶于碱液，能溶于稀酸，不溶于水和有机溶剂，难溶于醇。中性或淡酸性溶液暴露在紫外光中时，显强烈的亮蓝色荧光。
Appearance 黄色结晶粉末。易溶于碱液，能溶于稀酸，几乎不溶于水，难溶于醇。中性或淡酸性溶液暴露在紫外光中时，显强烈的亮蓝色荧光。熔点329-332℃
Stability对空气敏感
Boiling Point621.9°C at 760 mmHg
Flash Point329.9°C

别名和识别编码

Chemical NameLuminol
CAS Number521-31-3
Synonym Luminol {LY} Luminol {} {LY} Luminol {} {} {LY} Luminol {} {} {} {LY} Luminol {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Luminol {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Luminol
MDL NumberMFCD00006890
PubChem Substance ID87563135
Beilstein Registry Number383929
EC Number208-309-4
Reaxys-RN383929
Merck Number5600
Alfabeta NameAMINODIHYDROPHTHALAZINEDIONE 52314-----,,
Chemical Name Translation鲁米诺
LabNetwork Molecule IDLN00176012
InChIInChI=1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)
Canonical SMILESO=C1NNC(C2=C1C=CC=C2N)=O
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分类

  • {SNA} Biochemicals and Reagents, Chemiluminescent compounds, Enzyme Substrates, Luminescent Compounds/Detection, Peroxidase Substrates, Substrates by Enzyme, 酶、抑制剂和底物
  • {SNA} Biochemicals and Reagents, Bioelectronics, Biosensing and Bioimaging, Building Blocks, Chemical Synthesis, Chemiluminescent Dyes, Chemiluminescent compounds, Heterocyclic Building Blocks, Luminescent Compounds/Detection, Materials for Bioimaging, N-Containing, Organic and Printed Electronics, Others, Photonic and Optical Dyes, Photonic and Optical Materials, 材料科学
  • {SNA} Bioelectronics, Biosensing and Bioim

产品应用

  • 发光测试: Emmax 440 nm (化学发光; 60 mM K2S2O8, 100 mM K2CO3, pH 11.5; 添加H2O2后)
  • 化学发光试剂及指示剂，常用于化学发光分析，如金属阳离子、血液免疫等等。

相关文献及参考

  • Short: II/3
  • Merck: 14,5600
  • Merck: 14,5600 Beilstein:25(3/4)4192
  • Reagent for the generation of chemiluminescence.
  • Bull. Chem. Soc. Jpn. 65 , 1591, (1992)
  • Zhou, X., et al.: Anal. Chem. 81, 255 (2009),
  • Tang, D., et al.: Biosens. Bioelectron., 24, 2125 (2009),
  • Michalet, X., et al.: Chem. Rev., 106, 1785 (2006),

安全信息

WGK Germany3
GHS Symbol
Precautionary statements
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P264 Wash hands thoroughly after handling. 处理后要彻底洗净双手。
  • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
  • P302+P352+P332+P313+P362+P364
  • P305+P351+P338
  • P305+P351+P338+P337+P313
  • P362 Take off contaminated clothing and wash before reuse. 脱掉污染的衣服，清洗后方可重新使用
Hazard statements
  • H315 Causes skin irritation 会刺激皮肤
  • H319 Causes serious eye irritation 严重刺激眼睛
  • H335 May cause respiratory irritation 可能导致呼吸道刺激
Hazard Codes Xn Xi Xi,Xn
RTECSTH8890060
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
Signal word Warning
Safety Statements
  • S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后，立即用大量水冲洗并征求医生意见；
  • S36/37 Wear suitable protective clothing and gloves 穿戴适当的防护服和手套；
  • S36/37/39 Wear suitable protective clothing, gloves and eye/face protection 穿戴适当的防护服、手套和眼睛/面保护；
  • S37/39 Wear suitable gloves and eye/face protection 穿戴适当的手套和眼睛/面保护；
Risk Statements
  • R22 Harmful if swallowed 吞咽有害
  • R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
Warnings IRRITANT
Storage condition 2-8°C, protect from light {LY} 2-8°C, protect from light {} {LY} 2-8°C, protect from light {} {} {LY} 2-8°C, protect from light {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C, protect from light {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} 
TYPE OF TEST            : LD - Lethal dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : >500 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   NCNSA6 National Academy of Sciences, National Research Council,
   Chemical-Biological Coordination Center, Review.  (Washington, DC)
   Volume(issue)/page/year: 5,11,1953

TYPE OF TEST            : Sister chromatid exchange
TEST SYSTEM             : Rodent - hamster Lung
DOSE/DURATION           : 500 umol/L
REFERENCE :
   CHROAU Chromosoma.  (Springer-Verlag New York, Inc., Service Center, 44
   Hartz Way, Secaucus, NJ 07094)  V.1-    1939-  Volume(issue)/page/year:
   99,360,1990

其他信息

  • 鲁米诺价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/08/23 LA1459701 3-氨基邻苯二甲酰肼 5g 417元 2011/08/23 K0925302 3-氨基邻苯二甲酰肼 5g 753元 2011/08/23 SA851101 鲁米诺(3-氨基邻苯二甲酰肼) 5g 500元
  • 储运特性:库房通风低温干燥
  • 急性毒性:口服-大鼠LD:>;500 毫克/公斤
  • 毒性分级:中毒
  • 易溶于碱液，能溶于稀酸，不溶于水和有机溶剂，难溶于醇。中性或淡酸性溶液暴露在紫外光中时，显强烈的亮蓝色荧光。
  • 图谱信息:发光氨(521-31-3)红外图谱(IR2) 发光氨(521-31-3)红外图谱(IR1) 发光氨(521-31-3)质谱(MS) 发光氨(521-31-3)核磁图( 13 CNMR) 发光氨(521-31-3)核磁图( 1 HNMR)
  • 方法一:3-硝基苯二甲酸经环合，还原而得。将3-硝基苯二甲酸加入8%肼水溶液，
  • 化学荧光分子:鲁米诺是一种化学荧光分子，在过氧化氢分子存在的条件下可以转变成激发态氨基邻苯二甲酸，后者发出较强的荧光。过氧化氢是许多生物氧化反应的产物，因此很容易通过引入鲁米诺将这些生物氧化反应与光检测联系起来。例如用葡萄糖氧化酶/过氧化氢酶探头能够检测样品中的过氧化氢或葡萄糖的浓度，响应时间仅0.5s(动态法)。 将化学发光物质与免疫学反应结合起来，用光反应表示被测的免疫成分浓度，称为化学发光免疫分析技术(chemiluminescence immune assay，CLIA)。1976年Shmeder提出了化学发光免疫分析，以鲁米诺-H202及衍生物ABEI为检测反应显示系统，目前鲁米诺（luminol）及其衍生物已经是化学发光免疫测定应用中最常用的标记物，它们在碱性条件下，通过微过氧化物酶（microperoxidase）催化，可释放出大量光子，鲁米诺可用于HRP标记抗体的Western Blot和HRP标记探针的核酸杂交检测，也用于现代刑侦血迹检测。 化学发光物质能作为化学发光标记物的化合物须具备下列条件：发光的量子产率高；其理化特性与所研究的体系相匹配；其发光反应是发光物质氧化反应的结果；在所用的浓度范围内对生物体无毒。常用的几类化学发光试剂有：吖啶酯(acridinium ester)，是一种用途广泛的化学发光示踪物，它是一个三环的有机化合物，容易氧化，且氧化反应无需催化剂，在430nm处释放出光子；鲁米诺和异鲁米诺及其衍生物是最成熟的化学发光剂，早在1964年While就作了化学发光的研究报道，鲁米诺、
  • Sigma Aldrich:521-31-3(sigmaaldrich)
  • 化学发光法:化学发光法(Chemilum inescence)作为一种灵敏的测定方法被广泛用来检测酶、细胞及生物机体中产生的自由基及反应代谢物，其氧化产物和代谢产物所发出的光可用各种光度计检测。由于化学发光法具有灵敏、快速、操作简单和价格低廉等特点，而被广泛用于抗氧化剂的筛选和研究，如多酚、多糖、黄酮类、蒽醌类活性物质等。测定超氧阴离子常用的化学发光体系有黄嘌呤氧化酶-鲁米诺、邻苯三酚-鲁米诺及二甲基亚砜-鲁米诺三种体系，前者属于酶促体系，后两者则属于非酶促体系。测定
  • 化学发光法:化学发光法(Chemilum inescence)作为一种灵敏的测定方法被
  • 上游原料:水合肼 --> 连二亚硫酸钠 --> 氯化亚锡 --> 乙二醇 --> 碳酸铵 --> 对苯二甲酸 --> 3-硝基邻苯二甲酸 --> 杂环
  • 用途二:发光测试: Emma
  • 海
  • TCI Shanghai:3-氨基苯二甲酰肼[化学发光试剂] Luminol [Chemiluminescence Reagent],>;98.0%(T)(521-31-3)
  • MSDS 信息:3-Aminophthalhydrazide(521-31-3).msds
  • 用途一:作化学分析试剂、指示剂。
  • Alfa Aesar:3-氨基邻苯二甲酰肼,98% 3-Aminophthalhydrazide, 98%(521-31-3)

系列性分类

  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Luminescent Compounds/Detection - Biochemicals and Reagents
  4. Chemiluminescent compounds - Luminescent Compounds/Detection
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Fluorescent Probes, Labels, Particles and Stains - Biochemicals and Reagents
  4. Fluorescent Enzyme Substrates - Fluorescent Probes, Labels, Particles and Stains
  5. Fluorescent Enzyme Substrates for Spectroscopy - Fluorescent Enzyme Substrates
  1. Chemical Synthesis 化学合成
  2. Building Blocks 合成砌块
  3. Heterocyclic Building Blocks 杂环合成砌块
  4. Pyridazines 哒嗪
  1. Chemical Synthesis 化学合成
  2. Building Blocks 合成砌块
  3. Heterocyclic Building Blocks 杂环合成砌块
  4. Others 其他杂环
  1. Chemical Synthesis 化学合成
  2. Building Blocks 合成砌块
  3. Heterocyclic Building Blocks 杂环合成砌块
  4. Others 其他杂环
  5. N-Containing - Others
  1. Materials Science 材料科学
  2. Products for Organic & Printed Electronics Research
  3. Photonic and Optical Materials - Organic and Printed Electronics
  4. Chemiluminescent Dyes - Photonic and Optical Materials
  1. Analytical & Chromatography 分析化学与色谱
  2. Chemiluminescence
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  4. Fluorescent Enzyme Substrates
  5. Fluorescent Enzyme Substrates for Microscopy
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Enzymes, Inhibitors, and Substrates
  4. Enzyme Substrates
  5. Substrates by Enzyme
  6. Oxaloacetate decarboxylase
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Enzymes, Inhibitors, and Substrates
  4. Analytical Enzymes
  5. Peroxidase Enzymes and Reagents
  6. Peroxidase Inhibitors
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Fluorescent Probes, Labels, Particles and Stains
  5. Fluorescent Enzyme Substrates
  6. Fluorescent Enzyme Substrates for Microscopy