Trypan blue 台盼蓝

CAS 72-57-1 MFCD00003969

化学结构图

72-57-1
SMILES: Cc1cc(-c2ccc(/N=N/c3c(S(=O)(=O)[O-])cc4cc(S(=O)(=O)[O-])cc(N)c4c3O)c(C)c2)ccc1/N=N/c1c(S(=O)(=O)[O-])cc2cc(S(=O)(=O)[O-])cc(N)c2c1O.[Na+].[Na+].[Na+].[Na+]

化学属性

Mol. FormulaC34H24N6O14S4·4Na
Mol. Weight961
Melting Point300
TSCAYes
Solubility10 G/L (25 ºC)
Density1.007 g/mL at 20 °C
Appearance 蓝灰色粉状物，溶于水呈蓝色，微溶于溶纤纱，不溶于其他有机溶剂。遇浓硫酸为暗绿光蓝色，稀释后呈红光蓝色；遇浓硝酸呈棕光灰色溶液。其水溶液，遇10%硫酸为红色；遇氢氧化钠为红光紫色。

别名和识别编码

Chemical NameTrypan Blue
Synonym 2,7-Naphthalenedisulfonicacid,3,3’-[[3,3’-dimethyl(1,1’-biphenyl)-4,4’-diyl]bis(azo)]bis(5-amino 2,7-naphthalenedisulfonicacid,3,3’-((3,3’-dimethyl-4,4’-biphenylylene)bis(azo 3,3'-DIMETHYL-4,4'-(BIPHENYLENEBISAZO)BIS(8-AMINO-1-NAPHTHOL)-3,6-DISULFONIC ACID TETRASODIUM SALT 3,3'-{[3,3'-二甲基-(1,1'-二苯基)-4,4'-二基]双(偶氮)}-双(5-氨基-4-羟基-2, 4,4'-BIS(8-AMINO-3,6-DISULFO-1-HYD Azurro diretto BENZO BLUE 3B Benzamine Blue 3B Benzanil Blue R Benzo Blue Benzo Blue 3B Bleu Bleu diazole N 3B Bleue diretto 3B Blue 3B Blue, Diamine Blue, Niagara Blue, Trypan Brasilamina Blue 3B C.I. 23850 C.I. 23850; Trypan Blue; Niagara Blue 3B; Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydro {PubChem
MDL NumberMFCD00003969
CAS Number72-57-1
Beilstein Registry Number4360496
EC Number200-786-7
PubChem Substance ID6296
Merck Number9792
Chemical Name Translation台盼蓝
Reaxys-RN7125071
Wiswesser Line NotationL66J BQ DSWQ HSWQ JZ CNUNR B D- 2 &-NA- 4
LabNetwork Molecule IDLN01743703
InChIInChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
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分类

  • {SNA} In Vivo/In Vitro Tracking of Stem Cells,
  • {SNA} In Vivo/In Vitro Tracking of Stem Cells, Apoptosis and Cell Cycle, Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Core Bioreagents, Fluorescent reagen
  • {SNA} Microscopy Reagents, Stains and Dyes, 分析/色谱, 分析试剂
  • {SNA} Analytical Reagents, Analytical/Chromatography, Microscopy Reagents, Stains and Dyes
  • {SNA} Apoptosis and Cell Cycle, Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Other Apoptosis Related Reagents, Stains, Stains & Dyes, A to Z, Stains and Dyes, Stains, Dyes and Indicators, T-U-V, 细胞信号转导和神经科学, 细胞培养, 细胞生物学, 血液学和组织学, 试剂和添加剂

相关文献及参考

  • Merck: 14,9792
  • Merck: 14,9792 Beilstein:16(1)346

安全信息

Hazard statements
  • H226 Flammable liquid and vapour 易燃液体和蒸气
  • H350 May cause cancer 可能致癌
  • H351 Suspected of causing cancer 怀疑致癌
RTECSQJ6475000
GHS Symbol
Precautionary statements
  • P201 Obtain special instructions before use. 使用前获取专门指示。
  • P202 Do not handle until all safety precautions have been read and understood. 已阅读并理解所有的安全预防措施之前，切勿操作。
  • P203
  • P260 Do not breathe dust/fume/gas/mist/vapours/spray. 不要吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P271 Use only outdoors or in a well-ventilated area.? 只能在室外或通风良好的地方使用。
  • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
  • P281 Use personal protective equipment as required. 使用所需的个人防护装备。
  • P302+P352+P332+P313+P362+P364
  • P303+P361+P353
  • P308+P313
  • P318
  • P332+P313
  • P337+P313
  • P362 Take off contaminated clothing and wash before reuse. 脱掉污染的衣服，清洗后方可重新使用
  • P405 Store locked up. 上锁保管。
  • P501 Dispose of contents/container to..… 处理内容物/容器.....
WGK Germany3
Personal Protective Equipment Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
Signal word Danger
Safety Statements
  • S45 In case of accident or if you feel unwell seek medical advice immediately (show the label where possible) 发生事故时或感觉不适时，立即求医（可能时出示标签）；
  • S53 Avoid exposure - obtain special instructions before use 避免接触，使用前获得特别指示说明；
  • S60 This material and its container must be disposed of as hazardous waste 该物质及其容器必须作为危险废物处置；
  • S20 When using do not eat or drink 使用时，不得进食，饮水；
  • S36 Wear suitable protective clothing 穿戴适当的防护服；
Risk Statements
Hazard Codes T
Storage condition Store at RT. 密封阴凉干燥保存。
UN Number 2811 
TYPE OF TEST            : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 1275 mg/kg/34W-I
TOXIC EFFECTS :
   Tumorigenic - equivocal tumorigenic agent by RTECS criteria
   Liver - tumors
   Blood - leukemia
REFERENCE :
   APHGBP Acta Pathologica (Belgrade).  (Belgrade Univerzitet, Institute de
   Pathologie, Belgrade, Yugoslavia)  V.2/3-    1938- Volume(issue)/page/year:
   33,1,1953

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Parenteral
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 250 mg/kg/10W-I
TOXIC EFFECTS :
   Tumorigenic - equivocal tumorigenic agent by RTECS criteria
   Blood - lymphoma, including Hodgkin's disease
REFERENCE :
   CANCAR Cancer (Philadelphia).  (Lippincott/Harper, Journal Fulfillment
   Dept., 2350 Virginia Ave., Hagerstown, MD 21740)  V.1-    1948-
   Volu

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 630 mg/kg/43W-I
TOXIC EFFECTS :
   Tumorigenic - Carcinogenic by RTECS criteria
   Tumorigenic - tumors at site of application
REFERENCE :
   BJEPA5 British Journal of Experimental Pathology.  (Blackwell Scientific
   Pub. Ltd., POB 88, Oxford, UK)  V.1-    1920-  Volume(issue)/page/year:
   33,524,1952

TYPE OF TEST            : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 300 mg/kg/6W-I
TOXIC EFFECTS :
   Tumorigenic - equivocal tumorigenic agent by RTECS criteria
   Liver - tumors
   Blood - lymphoma, including Hodgkin's disease
REFERENCE :
   AJPAA4 American Journal of Pathology.  (Lippincott/Harper, Journal
   Fulfillment Dept., 2350 Virginia Ave., Hagerstown, MD 21740)  V.1-    19

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 328 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year:
   23,537,19

{haz

TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - guinea pig
DOSE/DURATION           : 300 mg/kg
TOXIC EFFECTS :
   Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
   PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
   (Academic Press, Inc., 1 E. First St., Duluth, MN 55802)  V.1-    1903/04-
   Volume(issue)/page/year: 31,825,1934

TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rabbit
DOSE/DURATION           : 100 mg/kg
TOXIC EFFECTS :
   Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
   PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
   (Academic Press, Inc., 1 E. First St., Duluth, MN 55802)  V.1-    1903/04-
   Volume(issue)/page/year: 31,825,1934

TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF E

TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 300 mg/kg
TOXIC EFFECTS :
   Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
   PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
   (Academic Press, Inc., 1 E. First St., Duluth, MN 55802)  V.1-    1903/04-
   Volume(issue)/page/year: 31,825,1934

{haza

TYPE OF TEST            : Body fluid assay
TEST SYSTEM             : Rodent - rat Bacteria - Salmonella typhimurium
DOSE/DURATION           : 500 mg/kg
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 156,131,1985

TYPE OF TEST            : Unscheduled DNA synthesis
TEST SYSTEM             : Rodent - hamster Liver
DOSE/DURATION           : 100 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 136,255,1984

{hazard_com

TYPE OF TEST            : Unscheduled DNA synthesis
ROUTE OF EXPOSURE       : Oral
TEST SYSTEM             : Rodent - rat
DOSE/DURATION           : 125 mg/kg
REFERENCE :
   ENMUDM Environmental Mutagenesis.  (New York, NY)  V.1-9, 1979-87.  For
   publisher information, see EMMUEG.  Volume(issue)/page/year: 5,488,1983

TYPE OF TEST            : Unscheduled DNA synthesis
TEST SYSTEM             : Rodent - rat Liver
DOSE/DURATION           : 10 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Nether

TYPE OF TEST            : DNA adduct
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 20 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 89,95,1981

TYPE OF TEST            : Cytogenetic analysis
ROUTE OF EXPOSURE       : Intraperitoneal
TEST SYSTEM             : Rodent - mouse
DOSE/DURATION           : 100 mg/kg
REFERENCE :
   CNJGA8 Canadian Journal of Genetics and Cytology.  (National Research
   Council of Canada, Publication Sales and Distribution, Ottawa ON K1A OR6,
   Canada)  V.1-    1959-  Volume(issue)/page/year: 10,91,1968

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - mouse
DOSE                    : 25 mg/kg
SEX/DURATION            : female 8 day(s) after conception
TOXIC EFFECTS :
   Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
   resorbed implants per total number of implants)
   Reproductive - Effects on Embryo or Fetus - fetot

TYPE OF TEST            : TDLo - Low

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Parenteral
SPECIES OBSERVED        : Rodent - rat
DOSE                    : 150 mg/kg
SEX/DURATION            : female 8-10 day(s) after conception
TOXIC EFFECTS :
   Reproductive - Specific Developmental Abnormalities - Central Nervous System
   Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
   JOANAY Journal of Anatomy.  (Cambridge Univ. Press, 32 E. 57th St., New
   York, NY 10022) V.51-    1916-  Volume(issue)/page/year: 100,361,1966

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE                    : 100 mg/kg
SEX/DURATION            : female 7 day(s) after conception
TOXIC EFFECTS :
   Reproductive - Specific Developmental Abnormalities - other developmental
   abnormalities
REFERENCE :
   JEZOAO Journal of Experimental Zoology.  (Alan R. Liss, Inc., 41 E. 11th
   St., New York, NY 10003) V.1-    1904-  Volume(issue)/page/year:
   171,343,1969

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE                    : 66667 ug/kg
SEX/DURATION            : female 8 day(s) after conception
TOXIC EFFECTS :
   Reproductive - Specific Developmental Abnormalities - Central Nervous System

其他信息

  • 蓝灰色粉状物，溶于水呈蓝色，微溶于溶纤纱，不溶于其他有机溶剂。遇浓硫酸为暗绿光蓝色，稀释后呈红光蓝色；遇浓硝酸呈棕光灰色溶液。其水溶液，遇10%硫酸为红色；遇氢氧化钠为红光紫色。
  • 图谱信息:3,3'-{[3,3'-二甲基-(1,1'-二苯基)-4,4'-二基]双(偶氮)}-双(5-氨基-4-羟基-2,7-萘二磺酸)四钠盐(72-57-1)红外图谱(IR1) 3,3'-{[3,3'-二甲基-(1,1'-二苯基)-4,4'-二基]双(偶氮)}-双(5-氨基-4-羟基-2,7-萘二磺酸)四钠盐(72-57-1)红外图谱(IR2)
  • 用途一:主要用于棉、麻、粘胶等纤维素纤维的染色，也可用于蚕丝、锦纶、粘/棉混仿纺的染色，还可用于纸张、皮革及生物染色。
  • 色指数:23850
  • 上游原料:硫酸钠
  • 方法一:由3，3'-二甲基联苯胺用亚硝酸钠与盐酸重氮化后，在碱性介质中与H酸进行偶合，而后经盐析、过滤及干燥而得。公斤/吨3，3'-二甲基联苯胺（100%） 100H酸（100%） 345亚硝酸钠（100%） 72纯碱 380盐酸（31%） 490精盐 2700元明粉 33
  • 海关编码:32041400
  • TCI Shanghai:直接蓝 14 Direct Blue 14(72-57-1)
  • Alfa Aesar:60% Trypan Blue, dye content >60%(72-57-1)" href="http://www.alfa.com/zh/GP100w.pgm?DSSTK=A18600&CAS;=72-57-1">台盼蓝,dyecontent>60% Trypan Blue, dye content >60%(72-57-1)
  • MOL 文件:72-57-1.mol
  • MSDS 信息:Direct Blue 14(72-57-1).msds
  • Acros Organics:锥虫蓝(台盼蓝) Trypan Blue(72-57-1)
  • Sigma Aldrich:72-57-1(sigmaaldrich)

系列性分类

  1. Life Science 生命科学
  2. Cell Biology 细胞生物学
  3. Bioactive Small Molecules - Cell Biology
  4. T - Bioactive Small Molecules
  1. Analytical & Chromatography 分析化学与色谱
  2. Analytical Reagents
  3. Microscopy Reagents - Analytical Reagents
  4. Stains and Dyes - Microscopy Reagents
  1. Life Science 生命科学
  2. Cell Culture 细胞培养
  3. Reagents and Supplements - Cell Culture
  4. Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability - Reagents and Supplements
  5. Stains, Dyes and Indicators - Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability
  1. Chemical Synthesis 化学合成
  2. Building Blocks 合成砌块
  3. Organic Building Blocks 有机砌块
  4. Nitrogen Compounds 氮化合物
  5. Azo/Diazo Compounds - Nitrogen Compounds
  1. Functional Group 官能团索引
  2. azo compound
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Biochemicals and Reagents
  4. Stains and Dyes
  5. Natural Dyes

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