3-(4,5-Dimethylthiazolyl)-2,5-diphenyl-2H-tetrazolium bromide 噻唑蓝

CAS 298-93-1 MFCD00011964

化学结构图

298-93-1
SMILES: [Br-].CC1N=C(SC=1C)[N+]1=NC(=NN1C1C=CC=CC=1)C1C=CC=CC=1

化学属性

Mol. FormulaC18
Mol. Weight414
Melting Point195
TSCAYes
Density1.422
Stability对光和湿度敏感
SolubilitySoluble in water (20 mg/ml), ethanol (20 mg/ml), 2-methoxyethanol (20 mg/ml), buffered salt solutions (5 mg/ml), culture media (5 mg/ml), and PBS.
Appearance powder

别名和识别编码

Chemical Name3-(4,5-Dimethylthiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
MDL NumberMFCD00011964
CAS Number298-93-1
Reaxys-RN3825277
PubChem Substance ID87567423
Synonym 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2h-tetrazoliubromide 2H-Tetrazolium,2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-,bromide 3,[4,5-DIMETHYLTHIAZOL-2-YL]-2,5-DIPHENYLTETRAZOLIUM BROMIDE 3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide 3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四氮唑鎓溴化物 Methylthiazolyldiphenyl-tetrazolium bromide THIAZOYL BLUE T Thiazolyl Blue Tetrazolium Bromide 噻唑蓝(MTT) 噻唑蓝溴化四唑;3-(4,5-二甲基-2-噻唑基)-2,5-二苯基溴化四唑;3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑噻唑蓝;2-(4,5-二甲基-2-噻唑基)-3,5-二苯基-2H-四唑氢溴酸盐;3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四氮唑鎓溴化物
EC Number206-069-5
Beilstein Registry Number4081397
Chemical Name Translation噻唑蓝
Wiswesser Line NotationT5NNKNJ BR& ER& C- BT5N CSJ D1 E1 &E
LabNetwork Molecule IDLN00197171
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分类

  • {SNA} In Vivo/In Vitro Tracking of Stem Cells, Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Cell Culture, Companion Products and Reagents, Core Bioreagents, General Stains/Dyes for Stem Cell Biology, General Stem Cell Biology, Insect Platform, Life Science Reagents for Cell Culture, Reagents and Supplements, Research Essentials,

产品应用

  • 常用于基于MTT检测方法的细胞活力、细胞增殖或细胞毒性检测。因为MTT具有正电荷，它可以穿过细胞膜并被线粒体还原形成紫色甲臜染料。溶解MTT甲臜染料需要使用有机溶剂。脱氢酶的检测，染色法。

相关文献及参考

  • 1. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65 , 55-63, (1983)
  • 2. Rastinejad., F., et al., Tumor suppression by RNA from the 3' untranslated region of α-tropomyosin. Cell 75 , 1107-17, (1993) 摘要
  • 3. Denizot, F., and Lang, R., Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89 , 271-7, (1986) 摘要
  • 4. Carmichael, J., et al., Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47 , 936-42, (1987)
  • 5. Boyle, V.J., et al., Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3 , 418-24, (1973) 摘要
  • 6. Pearse, A.G.E., and Hess, R., Subtantivity and other factors responsible for formazan patterns in dehydrogenase histochemistry. Experientia 17 , 136, (1961) 摘要
  • 7. Bernofsky, C., and Swan, M., An improved cycling assay for nicotinamide adenine dinucleotide. Anal. Biochem. 53 , 452-8, (1973) 摘要
  • 8. Hess, R., et al., Cytochemical localization of pyridine nucleotide-linked dehydrogenases. Nature 181 , 1531-2, (1958) 摘要
  • 9. Pearse, A.G., Intracellular localisation of dehydrogenase systems using monotetrazolium salts and metal chelation of their formazans. J. Histochem. Cytochem. 5 , 515-27, (1957) 摘要
  • Perri M, Pingitore A, Cione E, et al., Proliferative and anti-proliferative effects of retinoic acid at doses similar to endogenous levels in Leydig MLTC-1/R2C/TM-3 cells. Biochim. Biophys. Acta 1800 , 993-1001, (2010) 摘要
  • Beil. 27 ,IV,6045

安全信息

RTECSXF8060000
Safety Statements
  • S22 Do not breathe dust 不要吸入粉尘；
  • S24/25 Avoid contact with skin and eyes 避免皮肤和眼睛接触；
  • S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后，立即用大量水冲洗并征求医生意见；
  • S36/37 Wear suitable protective clothing and gloves 穿戴适当的防护服和手套；
  • S36/37/39 Wear suitable protective clothing, gloves and eye/face protection 穿戴适当的防护服、手套和眼睛/面保护；
  • S37 Wear suitable gloves 戴适当手套；
  • S60 This material and its container must be disposed of as hazardous waste 该物质及其容器必须作为危险废物处置；
Risk Statements
  • R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
  • R68 Possible risk of irreversible effects 可能有不可挽回的作用的危险
Storage condition 储存温度2-8℃,充氩保存 2-8℃避光保存。 Light Sensitive Light, Air & Moisture Sensitive 2-8°C
GHS Symbol
WGK Germany3
Hazard statements
  • H315 Causes skin irritation 会刺激皮肤
  • H341 Suspected of causing genetic defects 怀疑导致遗传性缺陷
  • H319 Causes serious eye irritation 严重刺激眼睛
  • H335 May cause respiratory irritation 可能导致呼吸道刺激
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Precautionary statements
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
  • P281 Use personal protective equipment as required. 使用所需的个人防护装备。
  • P305+P351+P338
Signal word Warning
Hazard Codes Xi Xn 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Salmonella typhimurium
DOSE/DURATION           : 5 ug/plate
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 68,107,1979

TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 5 ug/plate
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 68,107,1979

其他信息

  • Sigma Aldrich:298-93-1(sigmaaldrich)
  • TCI Shanghai:噻唑兰 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide,>;98.0%(T)(298-93-1)
  • Thiazolyl Blue is a membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT Formazan). Spectrophotometric measurement of MTT Formazan at 540 or 570 nm allows quantitation of cell viability. Thiazolyl Blue is used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. Thiazolyl Blue is also an electron acceptor used for studying NADP-dependent dehydrogenases.
  • Alfa Aesar:噻唑蓝溴化四唑,98% Thiazolyl Blue tetrazolium bromide, 98%(298-93-1)
  • 微溶于甲醇中，但难溶于水或乙醇中。用醚、丙酮或乙酸乙酯也不能将其完全溶解。195℃溶于水或DMSO。常配制成5mg/ml的储备液。
  • 海关编码:29341000
  • F:3-8-10
  • 噻唑兰价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/08/23 K8841501 噻唑蓝 1g 1120元 2011/08/23 SM212802 噻唑兰（+4℃） 1g 468元 2011/04/16 D0801 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基溴化四唑 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide 5G 3050元
  • MOL 文件:298-93-1.mol
  • MSDS 信息:3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(298-93-1).msds
  • 敏感性:Light Sensitive

系列性分类

  1. Research Essentials 基础科研
  2. Core Bioreagents - Research Essentials
  3. Stains and Dyes - Core Bioreagents
  1. Life Science 生命科学
  2. Hematology and Histology 血液学和组织学
  3. Stains and Dyes - Hematology and Histology
  4. Stains & Dyes, A to Z - Stains and Dyes
  5. T-U-V - Stains & Dyes, A to Z
  1. Life Science 生命科学
  2. Cell Culture 细胞培养
  3. Reagents and Supplements - Cell Culture
  4. Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability - Reagents and Supplements
  5. Stains, Dyes and Indicators - Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability
  1. Chemical Synthesis 化学合成
  2. Building Blocks 合成砌块
  3. Heterocyclic Building Blocks 杂环合成砌块
  4. Thiazoles 噻唑
  1. Chemical Synthesis 化学合成
  2. Building Blocks 合成砌块
  3. Heterocyclic Building Blocks 杂环合成砌块
  4. Thiazoles 噻唑
  1. Chemical Synthesis 化学合成
  2. Building Blocks 合成砌块
  3. Organic Building Blocks 有机砌块
  4. Nitrogen Compounds 氮化合物
  5. Azo/Diazo Compounds - Nitrogen Compounds
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Enzyme Substrates - Enzymes, Inhibitors, and Substrates
  5. Substrates by Enzyme - Enzyme Substrates
  6. Peroxidase Substrates - Substrates by Enzyme
  1. Life Science 生命科学
  2. Cell Biology 细胞生物学
  3. Stem Cell Biology - Cell Biology
  4. General Stem Cell Biology - Stem Cell Biology
  5. Stem Cell Characterization - General Stem Cell Biology
  6. General Stains/Dyes for Stem Cell Biology - Stem Cell Characterization
  1. Chemical Synthesis 化学合成
  2. Building Blocks 合成砌块
  3. Chemicals by Class 化学分类法
  4. Tetrazolium Salts & Formazans

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